Figure 1

Measured characteristics of cultures at steady-state. Limiting nutrients at bottom of figure: glucose (G), ammonium (N), phosphate (P), sulfate (S), leucine (L), uracil (U). Within each limitation, dilution rates are ordered from 0.05hr-1 to 0.3hr-1 in 0.05hr-1 increments. (A) Fraction of unbudded cells. (B) Steady-state culture density in Klett units. (C) Average cell volume. (D) Residual glucose and ethanol levels.

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